In this study, non-homogenous welding of nimonic 75 superalloy to Monel 400 with 1 mm thickness was investigated with pulsed Nd:YAG laser welding. The mechanical properties of the joint were analyzed with optical and scanning electron microscope, X-ray diffraction, micro-hardness test and tensile test. In the case of non-homogeneous welding of Nimoinc 75 superalloy to Monel 400, defects such as liquation cracks and porosity in the welded samples were observed. these defects were removed with increasing the preheating temperature and decreasing the heat input. The results showed the voltage, pulse width, pulse frequency and welding speed should be selected as 500 volts, 9 milliseconds, 3 Hz and 0.9 mm/s respectively to reach the proper penetration depth. Also, the investigations show that the welding structure is composed of austenitic matrix containing columnar dendrites and some cellular areas. The mechanical properties of the weld metal were reduced after joining and segregation causes a change in the amount of elements and the appearance of intermetallic compounds in the spaces between dendrites and cells. All non-homogeneous samples broke during the tensile test from the weld metal area.